Abstract: FISH technology was a common method to detect the nucleic acid sequence of malignant tumor cells. It was widely used in the field of cancer diagnoses and treatments. In order to realize the full automation of FISH experiments, a full-automatic pathological staining system was designed, which was provided with multi axis control arm, reagent sampler, slide, cover slide fixture, auxiliary module and so on. The positioning erorrs of the multi axis control arm are less than ±0.1 mm, the accuracy of extracting and discarding the pipette head is more than 99.5%, the success rate of extracting the cover glass is more than 99%, and the erorrs of micro reagent sampling are less than 0.6 μL. The errors of adding a large amount of reagent are less than 0.5 mL, the temperature control erorrs of slide clamp are less than ±1 ℃, and there is no reagent leakage. The results of biological experiments show that the system has good fluorescence staining effectiveness, the signal points are clearly visible, and the interpretable rate of fluorescence image is more than 90%.

Key words: fluorescence in situ hybridization(FISH), full-automatic pathological staining system, mechanical structure, motion module, fixture module

摘要： 荧光原位杂交(FISH)技术是检测恶性肿瘤细胞核酸序列的常用方法，在癌症的诊疗领域有广泛应用。为实现FISH实验的全自动化，设计了一种全自动病理染色系统。该系统设置有多轴操纵臂、试剂加样器、载玻片、盖玻片夹具、辅助模块等。多轴操纵臂定位误差不大于±0.1 mm，提取及丢弃移液枪头准确率大于99.5%，抽取盖玻片成功率大于99%，微量试剂加样误差不大于0.6 μL，大量试剂加样误差不大于0.5 mL，载玻片夹具控温误差不大于±1 ℃且无试剂泄漏。生物实验结果表明，该系统荧光染色效果良好，信号点清晰可见，荧光图像可判读率超过90%，具有较好的人工替代性。

关键词: 荧光原位杂交(FISH), 全自动病理染色系统, 机械结构, 运动模块, 夹具模块