关键词: 显微注射；数字化进退针；快速刺膜；压电陶瓷；实验研究

Abstract:

To solve the problems of low speed and precision of micropipette movement, a novel digital piercing and withdrawing micropipette device (DPWMD) was
presented for microinjection. By piezo-ceramic actuators’ low frequency vibration,
impact inertial force driven by controllable pulse sequence was produced.On the drive of inertial force and friction force alternately, the micro-motion was generated by the DPWMD,
step by step. The moving performance of DPWMD was tested. Digital piercing and with drawing micropipette device was applied in mouse oocyte microinjection experiment. The motion precision came to nanometer.The processing parameters were digitally controllable.The DPWMD can automatically complete micromanipulation, including piercing cell membrane speedily,penetrating into cellular some areas accurately,and withdrawing micropipette rapidly.

Key words: microinjection;digital piercing and withdrawing micropipette;pierce