关键词: 显微注射；数字化进退针；穿刺细胞膜；小鼠卵母细胞；实验研究

Abstract:

In order to resolve the prolems of low penetrating speed and positioning precision of micropipettes in microinjection, a novel digital piercing and withdrawing micro-pipette device(DPWMD) was presented for cell engineering. The DPWMD was alternately actuated by inertial force and friction force. Using DPWMD, an experimental system for digital microinjection was developed primarily. With experimental animal of Kunming mouse, the digital microinjection experiments were executed for mouse oocytes. With estimating criterions of cell distortion ratio and piercing efficiency, the influences on experimental efficiency were respectively analyzed with change of DPWMD’s drive voltage and frequency. The control parameters of DPWMD were optimized by experiments. With analysis of the result data, the successful rate is improved remarkably in the digital microinjection experiments of mouse oocyte. Microinjection experimental efficiency comes to 149 oocytes per hour. The digital piercing and withdrawing micropipette device can fully meet the needs of microinjection.

Key words: microinjection, digital piercing and withdrawing micropipette, piercing cellular plasma membrane, mouse oocyte, experimental research